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KMID : 0861020180330060071
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Korea Journal of Herbology
2018 Volume.33 No. 6 p.71 ~ p.78
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Anti-inflammatory Effects of Ethanol Extract of Chinese Medicinal Plantsin Yanjin on LPS-stimulated RAW 264.7 Macrophages
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Park Yea-Jin
Seo Jong-Hwan
Gil Tae-Young
Cheon Se-Yun
Piao Ren-Zhe
Lee Sang-Woo
Cha Yun-Yeop
An Hyo-Jin
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Abstract
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Objectives : This study was fulfilled to investigate nominee materials as anti-inflammatory agent from ethanol extract of Chinese medicinal plants in Yanjin. Among the 20 candidates, we selected most effective one, the ethanol extract of Cicuta virosa L. (CVL). The mechanism underlying the anti-inflammatory effects of CVL is not clearly identified as yet. Accordingly, we clarified the anti-inflammatory effects of CVL and its underlying molecular mechanisms in LPS-stimulated RAW 264.7 macrophages.
Methods : RAW264.7 macrophages were incubated with CVL (12.5, 25, or 50 M) and/or lipopolysaccharide (LPS) (1 ㎍/㎖). Cytotoxicity was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the level of nitric oxide (NO) production was measured with Griess reagent. The prostaglandin E2 (PGE2) production was measured with enzyme immunoassay kits and the protein expression of inducible nitric oxide synthase (iNOS) was determined using Western blot analysis.
Results : Among the 20 ethanol extract of Chinese medicinal plants of Yanjin tested, CVL significantly reduced the production of NO in a dose-dependent manner via inhibition the protein expressions of iNOS without cytotoxicity on the LPS-stimulated RAW 264.7 macrophages. In addition, CVL also effectively declined the production of PGE2 in LPS-simulated RAW 264.7 macrophages.
Conclusions : Taken together, these data presented in this study demonstrate that CVL possesses anti- inflammatory activity by suppressing the production of pro-inflammatory mediators NO and PGE2, and pro- inflammatory protein iNOS expression in LPS-stimulated RAW 264.7 macrophages.
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KEYWORD
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Cicuta virosa L., lipopolysaccharide, nitric oxide, prostaglandin E2, inducible nitric oxide synthase
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